Three-dimensional Reconstruction of Large Tissue Volumes From Scanning Laser Confocal Microscopy
نویسندگان
چکیده
Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.
منابع مشابه
Application of Phase Correlation to the Montage Synthesis and 3D Reconstruction of Large Tissue Volumes from Confocal Laser Scanning Microscopy Brief title: Montage Synthesis via Phase Correlation
We have implemented and tested a new automatic method for the montage synthesis and 3D reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for C...
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